In general, a web application can render any HTML it likes. Therefore, in theory, any language could render identical HTML output.
However, there are some ways we can try to deduce what is running server-side. For instance, file extensions are usually a dead giveaway (although they could technically be faked). Due to my experience with ASP.NET, I know that one way we can identify an ASP.NET application is by the presence of VIEWSTATE in the rendered document, although the lack of viewstate does not necessarily mean that the application is not running on ASP.NET
We could run some OS/server fingerprinting on the server, but in general that would not help much - these days, even Windows servers can run quite a few application platforms.
What other analysis techniques are available that can help us determine what's running on the server? What other clues do certain languages leave?
If we can better understand the artifacts our applications are leaving, perhaps so small that we haven't noticed them, we can begin to better our security by removing them from the output.