Yes, of course. If you want just to call a wcf service with a callback (Duplex) you can do it as usual in the script method.
Edit:
I don't know what you have to do with Ajax, but, suppose that you want to suggest the user to fill a textbox with an AutoCompleteExtender using data coming from a WCF service, you have to provide a ScriptMethod. Inside it you can call the service in the usual way as explained in MSDN
[WebMethod, ScriptMethod]
public static string[] GetSuggestedItems(string prefixText, int count)
{
// Construct InstanceContext to handle messages on callback interface
InstanceContext instanceContext = new InstanceContext(new MyCallbackHandler());
// Create a client
MyDuplexClient client = new MyDuplexClient(instanceContext);
return client.GetModelItems(prefixText).GetRange(0, count);
}
once you have defined callback handler:
public class MyCallbackHandler : IMyCallbackContract
{
....
}