This would be appropriate here:
"It takes considerable knowledge just to realize the extent of your own ignorance."
-Thomas Sowell
So, when struggling to get a Flash-based, JavaScript-configured component to work in my web app, I added a staticContent node to my web.config, with a mimeMap node as a child:
<configuration>
...
<system.webServer>
...
<staticContent>
<mimeMap fileExtension=".mp4" mimeType="video/mpeg" />
</staticContent>
</system.webServer>
</configuration>
When I commented-out the entire staticContent node, everything worked just fine. I didn't know that adding a mimeMap here would cause all of the default mimeMaps (specified within the server's ApplicationHost.config) to be overridden, because that seems to be exactly what is going on...Then again, I am merely guessing - either way, not very easy to figure out.
Thank you to everyone that responded, I appreciate it!