I don't entirely agree with the reasoning saying "it may confuse".
To some extent I can see what is meant, that there is no reason to venture outside "common understanding" -- everybody understands object != null.
But in Visual Studio, we have wonderful tools where you can simply hover over the method, to reveal some additional information.
If we were to say that the extension-method was annotated with a good explanation, then I feel that the argument of confusion falls apart.
The methods .IsNotNull() and .IsNull() explain exactly what they are. I feel they are very reasonable and useful.
In all honesty it is a matter of "what you like". If you feel the methods will make it more readable in the context of your project, then go for it. If you are breaking convention in your project, then I would say the opposite.
I have had the same thoughts as you have on the subject and have asked several very very experienced developers at my place of work. And none of them have come up with a good reason (except what has been mentioned about -confusion- here) that would explain why you shouldn't do this.
Go for it :-)