Ninject Framewor. It is a great fluent Dependancy Injection Framework. It is lighter weight and more very quick. I fell in love with it the more I use it in the ASP.NET MVC projects I am working on.
ASP.NET MVC - its about time, and boy are they doing it right.
jQuery - not really a 3rd part Framework for ASP.NET, but its a great compliment to ASP.NET and honestly I couldnt do my job without it.
MS Ajax Client Templates - If you havent checked this out yet, do yourself a favor and check it out. It is a way to bind Javascript Objects to controls and have live templating, so it a 2-way bind like in Silverlight and WPF. Its a great javascript library, that will hopefully get better and more mature.
If anyone is interested in these things, check out my latest 2 blog posts at polymorphicview.blogspot.com to see how I am using them together.