It all depends on your audience.
If most of your audience is going to be fairly hip web developers that know to use a decent browser, you are probably going to be fine using HTML5.
However, if your audience is any government institution (school, etc), business place, etc, you might not want to use it yet. My school currently runs on Internet Explorer 6/7, and one of my teacher constantly complains about that "Your browser is not supported" message at the top of Youtube. These people probably don't have any control over the browser they use, and might be a bit behind due to the IT guys.
Find your audience, and use what you are comfortable using with them.