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I am using windows authentication without impersonation on my company's intranet website with IIS7.

Under IIS7, what account is used to access the folder which contains my web app using these settings?

Would it be IIS_IUSRS? Or NETWORK SERVICE? Or another I don't know about?

+1  A: 

In IIS 7 (not IIS 7.5), sites access files and folders based on the account set on the application pool for the site. By default, in IIS7, this account is NETWORK SERVICE.

Specify an Identity for an Application Pool (IIS 7)

In IIS 7.5 (Windows 2008 R2 and Windows 7), the application pools run under the ApplicationPoolIdentity which is created when the application pool starts. If you want to set ACLS for this account, you need to choose APPPOOL\ApplicationPool instead of NT Authority\Network Service.

Thomas