I would split() the string and check an individual component of the url:
var str = "http://store.mywebsite.com/folder-1/folder-2/item3423434.aspx"
// split the string into an array of parts
var spl = str.split("/");
// spl is now [ http:,,store.mywebsite.com,folder-1,folder-2,item3423434.aspx ]
if (spl[4] == "folder-1") {
// do something
} else if (spl[4] == "folder-2") {
// do something else
}
Using this method it's easy to check other parts of the URL too, without having to use a regular expression with sub-expression captures. e.g. matching the second directory in the path would be if spl[5] == "folder-x".
Of course, you could also use indexOf(), which will return the position of a substring match within a string, but this method is not quite as dynamic and it's not very efficient/easy to read if there are going to be a lot of else conditions:
var str = "http://store.mywebsite.com/folder-1/folder-2/item3423434.aspx"
if (str.indexOf("http://store.mywebsite.com/folder-1") === 0) {
// do something
} else if (str.indexOf("http://store.mywebsite.com/folder-2") === 0) {
// do something
}