The bundled documentation for the read
command, reproduced here, provides a lot of information that is relevant to your question.
read FILEHANDLE,SCALAR,LENGTH,OFFSET
read FILEHANDLE,SCALAR,LENGTH
Attempts to read LENGTH characters of data into variable SCALAR
from the specified FILEHANDLE. Returns the number of
characters actually read, 0 at end of file, or undef if there
was an error (in the latter case $! is also set). SCALAR will
be grown or shrunk so that the last character actually read is
the last character of the scalar after the read.
An OFFSET may be specified to place the read data at some place
in the string other than the beginning. A negative OFFSET
specifies placement at that many characters counting backwards
from the end of the string. A positive OFFSET greater than the
length of SCALAR results in the string being padded to the
required size with "\0" bytes before the result of the read is
appended.
The call is actually implemented in terms of either Perl's or
system's fread() call. To get a true read(2) system call, see
"sysread".
Note the characters: depending on the status of the filehandle,
either (8-bit) bytes or characters are read. By default all
filehandles operate on bytes, but for example if the filehandle
has been opened with the ":utf8" I/O layer (see "open", and the
"open" pragma, open), the I/O will operate on UTF-8 encoded
Unicode characters, not bytes. Similarly for the ":encoding"
pragma: in that case pretty much any characters can be read.