If you use SysInternals' ZoomIt utility, you can see that this is simply two lines. A dark gray one above a white one. Drawing lines is simple enough with Graphics.DrawLine(), you just need to make sure you pick a dark color that work well with the form's BackColor. That isn't always battleship gray if the user selected another theme. Which makes the GroupBox trick fall flat.
This sample code is serviceable:
protected override void OnPaint(PaintEventArgs e) {
Color back = this.BackColor;
Color dark = Color.FromArgb(back.R >> 1, back.G >> 1, back.B >> 1);
int y = button1.Bottom + 20;
using (var pen = new Pen(dark)) {
e.Graphics.DrawLine(pen, 30, y, this.ClientSize.Width - 30, y);
}
e.Graphics.DrawLine(Pens.White, 30, y+1, this.ClientSize.Width - 30, y+1);
}
Note the use a button1 in this code, there to make sure the line is drawn at the right height, even when the form is rescaled. Pick your own control as a reference for the line.