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If the website's physical directory is not in C:\inetpub\wwwroot and is instead in C:\MyProjects\Project1 - which account needs what rights to the MyProjects or the Project1 folder?

I get a security error when I browse to the website.

A: 

It's usually the group YOURMACHINE\IIS_IUSRS which contains all the default app pool users. If you're using a domain user for the app pool then it'll be that instead.

They'll need at least read and list folder contents - and I expect Read & Execute too although I'm not 100%. I usually leave that on as it's in the default set of read permissions. If you have a SQL Express database in the tree then presumably they'll need permission to write to that too, etc.

Rup