On the ASP.NET side of things, you can create the DropDownList with AppendDataBoundItems="true" and any items you bind to it will come after the default:
<asp:DropDownList AppendDataBoundItems="true" ID="yourListId" runat="server">
<asp:ListItem Text="Select something" Value="-1" />
</asp:DropDownList>
As for doing the same thing completely in Javascript, you can do it with a function like this:
function addFirstItem(list, text, value)
{
var newOption = document.createElement("option");
newOption.text = text;
newOption.value = value;
list.options.add(newOption);
}
addFirstItem(document.getElementById("yourListId"), "Select something", "-1");
Or with jQuery (there is probably something much cleaner, especially for creating a new option tag, but this works):
$("#yourListId option:first").before("<option value='-1'>Select something</option>");