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I have 2 app pools one for asp.net 2.0 one for asp.net 4.0

does doing an iisreset recycle these pools? or do I have to recycle them by hand?

+1  A: 

An IISReset will recycle all your application pools, restart some services, etc.

Erik Noren
A: 

IISReset stops and restarts the entire web server (including non-ASP.NET apps) Recycling an app pool will only affect applications running in that app pool. Editing the web.config in a web application only affects that web application (recycles just that app). Editing the machine.config on the machine will recycle all app pools running.

IIS will monitor the /bin directory of your application. Whenever a change is detected in those dlls, it will recycle the app and re-load those new dlls. It also monitors the web.config & machine.config in the same way and performs the same action for the applicable apps.

drooksy