Don't be scared of 1-line functions!
A lot of programmers seem to have a mental block about 1-line functions, you shouldn't.
If it makes the code clearer and cleaner, extract the line into a function.
Performance probably won't be affected.
Any decent compiler made in the last decade (and perhaps further) will automatically inline a simple 1-line function. Also, 1-line of C can easily correspond to many lines of machine code. You shouldn't assume that even in the theoretical case where you incur the full overhead of a function call that this overhead is significant compared to your "one little line". Let alone significant to the overall performance of your application.
Abstraction Leads to Better Design. (Even for single lines of code)
Functions are the primary building blocks of abstract, componentized code, they should not be neglected. If encapsulating a single line of code behind a function call makes the code more readable, do it. Even in the case where the function is called once. If you find it important to comment one particular line of code, that's a good code smell that it might be helpful to move the code into a well-named function.
Sure, that code may be 1-line today, but how many different ways of performing the same function are there? Encapsulating code inside a function can make it easier to see all the design options available to you. Maybe your 1-line of code expands into a call to a webservice, maybe it becomes a database query, maybe it becomes configurable (using the strategy pattern, for example), maybe you want to switch to caching the value computed by your 1-line. All of these options are easier to implement and more readily thought of when you've extracted your 1-line of code into its own function.
Maybe Your 1-Line Should Be More Lines.
If you have a big block of code it can be tempting to cram a lot of functionality onto a single line, just to save on screen real estate. When you migrate this code to a function, you reduce these pressures, which might make you more inclined to expand your complex 1-liner into more straightforward code taking up several lines (which would likely improve its readability and maintainability).