Depends on what you call use and on what you call mono...
I can confirm it works with mono on Windows.
I can confirm that when you remove system.linq and system.xml from the web.config, it will run on VB.NET on Linux under mono. It will run on C# under Linux without modifiying the web.config.
But in both cases (on Linux) you have to use RenderType="BinaryStreaming", because else, it uses a temporary directory where it generates the chart image. The problem with that is it only accepts \ as directoryseparator (checks the tmp directory in the web.config file on startup), and Linux uses /, so you aren't able to get it up and running with any Linux temp directory, that's why you need to select BinaryStreaming..
But appart from that, it works wonderfully.
However, ZedChart is the better option anyway (has more options), except that ZedChart doesn't support any kind of 3d-chart, yet.