Hmm... Depending on the exact version of Regex you're running, consider:
(?P<id>\d{3})(?=[A-Za-z\s]{16}\d)(?P<username>[A-Za-z]{8,16})\s*(?P<phone>\d{8})
Note 100% sure this will work, and I've used the whitespace escape char instead of an actual space - I get nervous with just the space character myself, but you may want to be more restrictive.
See if it works. I'm only intermediate with RegEx myself, so I might be in error.
Check out the named groups syntax for your version of RegEx a) exists and b) matches the standard I've used above.
EDIT:
Just to expand what I'm trying to do (sorry to make your eyes bleed, Pax!) for those without a lot of RegEx experience:
(?P<id>\d{3})
This will try to match a named capture group - 'id' - that is three digits in length. Most versions of RegEx let you use named capture groups to extract the values you matched against. This lets you do validation and data capture at the same time. Different versions of RegEx have slightly different syntaxes for this - check out http://www.regular-expressions.info/named.html for more detail regarding your particular implementation.
(?=[A-Za-z\s]{16}\d)
The ?= is a lookahead operator. This looks ahead for the next sixteen characters, and will return true if they are all letters or whitespace characters AND are followed by a digit. The lookahead operator is zero length, so it doesn't actually return anything. Your RegEx string keeps going from the point the Lookahead started. Check out http://www.regular-expressions.info/lookaround.html for more detail on lookahead.
(?P<username>[A-Za-z]{8,16})\s*
If the lookahead passes, then we keep counting from the fourth character in. We want to find eight-to-sixteen characters, followed by zero or more whitespaces. The 'or more' is actually safe, as we've already made sure in the lookahead that there can't be more than sixteen characters in total before the next digit.
Finally,
(?P<phone>\d{8})
This should check the eight-digit phone number.
I'm a bit nervous that this won't exactly work - your version of RegEx may not support the named group syntax or the lookahead syntax that I'm used to.
I'm also a bit nervous that this Regex will successfully match an empty string. Different versions of Regex handle empty strings differently.
You may also want to consider anchoring this Regex between a ^ and $ to ensure you're matching against the whole line, and not just part of a bigger line.