So if I understand correctly you no longer specify the AttachDBFilename but instead you have attached the database 'for real' to an existing SQL Server instance.
since you are no longer conencting to your own personal RANU instance, your application must have proper credentials to connect to the SQL Server instance where you attached the database. The correct solution depends on a number of factors, but possible answers are:
- create a SQL Server login for the ASP app pool identity and grant this loggin proper access to the required database. Use CREATE LOGIN [domain\user] FROM WINDOWS and CREATE USER [domain\user]. Better still, for extra credit, add the app pool identity to a security group and grant this security group the needed permission.
- change the app pool identity to an indetity that has the proper permissions already granted
- if the ASP application uses impersonation and the SQL Server instance is on a different machine from the ASP application, make sure your ASP app pool is allowed to do constrained delegation.