Yes, of course you can - just require Windows credentials (i.e. an Active Directory account in your domain) from your callers.
Anyone not authenticated against your domain will be rejected.
You can do this by specifying either netTcpBinding with transport security (if everything is behind a corporate firewall), or wsHttpBinding with message security:
<bindings>
<netTcpBinding>
<binding name="DomainUsersOnly">
<security mode="Transport">
<transport clientCredentialType="Windows" />
</security>
</binding>
</netTcpBinding>
<wsHttpBinding>
<binding name="HttpDomainUsersOnly">
<security mode="Message">
<message clientCredentialType="Windows" />
</security>
</binding>
</wsHttpBinding>
</bindings>
Now, all you need to do is reference one of those binding configurations in your endpoints:
<endpoint name="whatever"
address="......"
binding="netTcpBinding"
bindingConfiguration="DomainUsersOnly"
contract="IYourservice" />
and you should be good to go.