+1  A: 

I am able to solve the problem, by moving python script outside htdocs

WSGIScriptAlias /wsgi "C:/wsgi/"

<Directory "C:/wsgi">
    AllowOverride None
    Options None
    Order deny,allow
    Allow from all
</Directory>
Yan Cheng CHEOK
That should have problems as well as when mapping to a directory the trailing slash on URL where it mounts in WSGIScriptAlias line is important.
Graham Dumpleton