Locking is a possible solution, but I prefer the general architecture of having each external resource (including a file) dealt with by a single, separate thread. Other threads send work requests to the dedicated thread on a Queue.Queue instance (and provide a separate queue of their own as part of the work request's parameters if they need result back), the dedicated thread spends most of its time waiting on a .get
on that queue and whenever it gets a requests goes on and executes it (and returns results on the passed-in queue if needed).
I've provided detailed examples of this approach e.g. in "Python in a Nutshell". Python's Queue is intrinsically thread-safe and simplifies your life enormously.
Among the advantages of this architecture is that it translates smoothly to multiprocessing if and when you decide to switch some work to a separate process instead of a separate thread (e.g. to take advantage of multiple cores) -- multiprocessing
provides its own workalike Queue
type to make such a transition smooth as silk;-).