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answers:

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I need to read in about 1KB or so of data at a time, manipulate it, and write it to another file. I need to do this for at least 100 MB. I have never done any file IO in assembly before.

What interrupts do I need to call and what needs to be in what registers?

A: 

Please specify the OS you're targeting. For DOS, google for Ralf Brown's Interrupt List. For linux, look for asm/unistd.h.

ninjalj