Hi I have two images of yeast plates:
Permissive:
Xgal:
The to images should be in the same spot and roughly the same size. I am trying to use one of the images to generate a grid and then apply that grid to the other image. The grid is made by looking at the colonies on permissive plate, the plate should have 1536 colonies on it. The problem is that the camera that was used to take the images moves a bit up and down and the images can also be shifted slightly due to the other plate not being in exactly the same place. This then means that when I use the permissive plate to generate the grid on the xgal plate the grid shifts. Does anyone know a way in which I can compensate for this? I am using perl with the gd module. Any advice would be greatly appreciated. Thank you