Thank you for all the replies. This is my source code..
asp:Accordion ID="sample_accordion1"
CssClass="accordion"
HeaderCssClass="accordionHeader"
HeaderSelectedCssClass="accordionHeaderSelected"
ContentCssClass="accordionContent"
runat="server"
SelectedIndex="0"
FramesPerSecond="30"
TransitionDuration="200"
AutoSize="None">
<Panes>
<asp:AccordionPane ID="AccordionPane1" runat="server">
<Header>Accordion 1</Header>
<Content>
Some text..................
</Content>
</asp:AccordionPane>
<asp:AccordionPane ID="AccordionPane2" runat="server">
<Header>Accordion 2</Header>
<Content>
Some text here......................
</Content>
</asp:AccordionPane>
<asp:AccordionPane ID="AccordionPane3" runat="server">
<Header>Accordion 3</Header>
<Content>
Some text here.................
</Content>
</asp:AccordionPane>
</Panes>
</asp:Accordion>
And this code is not working in my scenerio. Only one accordianPane is open(1st one). And I couldn't find any errors...Is this a problem related with my ajax toolkit installation ? I am using VS2008. And Ajax toolkit version 3.5. I am just biginner in ajax controls. :(