There are a few ways of doing this and it depends on what your environment is. If you're using Spring there's a fair chance you're deploying a Web application or you're otherwise in a J2EE environment. If this is the case (and arguably even if it isn't) you probably want to configure a DataSource.
This is a fairly minimal solution:
<bean id="dataSource" class="org.apache.commons.dbcp.BasicDataSource" destroy-method="close">
<property name="driverClassName" value="${jdbc.driverClassName}"/>
<property name="url" value="${jdbc.url}"/>
<property name="username" value="${jdbc.username}"/>
<property name="password" value="${jdbc.password}"/>
</bean>
The above is using the Apache (Jakarta Commons) database connection pooling but your appserver probably has an alternative you may want to use instead. Also, different database vendors have their own data source implementations too (eg OracleDataSource and OracleXADataSource for Oracle).
Note the use of properties like jdbc.username. This is a typical configuration because database configurations typically vary between environment. You can activate a property configurator with something like:
<bean id="jdbcConfiguration" class="org.springframework.beans.factory.config.PropertiesPlaceholderConfigurer">
<property name="location" value="classpath:jdbc.properties"/>
</bean>
Now you probably want transactions too I would imagine. The easiest way is to use a platform transaction manager but, like with most things Spring, there are multiple ways of doing it.
<bean id="txManager" class="org.springframework.jdbc.datasource.DataSourceTransactionManager">
<property name="dataSource" ref="dataSource"/>
</bean>
After this you can use this bean directly or (arguably more common) you can use declarative transactions with AOP (annotations).
More on these subjects in the (superb) Spring reference documentation.