If you are using SQL Developer, Standard, or any other version aside from Express you need to attach the database to the SQL server before you can use it. In SQL management studio connect to your server, right click on the Databases folder and choose attach, browse to your database and select it (note that you may need to move it to a directory SQL can see - by default SQL runs as Network Service and cannot peer inside C:\Users).
Once you've done that you need to tell ASP.NET that is the database you wish to use. There are a few ways to do this but by fair the easiest is to override the SQL Express database connection by adding the following to your web.config
<connectionStrings>
<remove name="LocalSqlServer" />
<add name="LocalSqlServer" connectionString="Data Source=myServerAddress;Initial Catalog=myDataBase;Integrated Security=SSPI;" providerName="System.Data.SqlClient"/>
</connectionStrings>
Don't forget to give Network Service, or whatever identity you run your application pool as, access to the database in SQL server.