The main drawback of GUI builders is that they make it easy to do simple things and very difficult to do complicated things. It's the same as building a website with a real design tool vs. using a simple website builder like iWeb or FrontPage.
The three most important topics in GUI framework are learning the event model, the layout model, and the intricacies of each widget. GUI builders isolate you from the latter two and kind of restrict your exposure to your first.
In the long run, it's detrimental to the things you can achieve, and therefore detrimental to your mastering of GUI creation.
In addition, maybe behaviors that are very important to the users (e.g., blanking out one widget in response to change in another) are quite tricky to write, especially with automatically generated code. Complex GUIs often rely on models, and GUI builders often through everything together, so it's much easier to end up with horrible unmaintainable code.