This is what you need for ASP.NET 4.0 / IIS 7.5 on Windows 7:
Your web.config must contain the following:
<appSettings>
<add key="ChartImageHandler" value="storage=file;timeout=20;" />
</appSettings>
<compilation targetFramework="4.0">
<assemblies>
<add assembly="System.Web.DataVisualization, Version=4.0.0.0, Culture=neutral, PublicKeyToken=31BF3856AD364E35"/>
</assemblies>
</compilation>
<system.webServer>
<handlers>
<add name="ChartImg" verb="*" path="ChartImg.axd" type="System.Web.UI.DataVisualization.Charting.ChartHttpHandler, System.Web.DataVisualization, Version=4.0.0.0, Culture=neutral, PublicKeyToken=31bf3856ad364e35" />
</handlers>
</system.webServer>
You also need this at the top of your aspx page:
<%@ Register Assembly="System.Web.DataVisualization, Version=4.0.0.0, Culture=neutral, PublicKeyToken=31bf3856ad364e35"
Namespace="System.Web.UI.DataVisualization.Charting" TagPrefix="asp" %>
Hope this helps