Recent versions of PHP have a cache of filenames for knowing the real path of files, and require_once() and include_once() can take advantage of it.
There's a value you can set in your php.ini to set the size of the cache, but I have no idea how to tell what the size should be. The default value is 16k, but I see no way of telling how much of that cache we're using. The docs are vague:
Yes, I can jack up the amount of cache allowed, and run tests with ab or some other testing, but I'd like something with a little more introspection than just timing from a distance.