In my opinion there are two alternatives (on Windows):
- Profilers that change the assembly instructions of your applications (that's called instrumenting) and record every detail. These profilers tend to be slow (applications running about 10 times slower), sometimes hard to set up, and often not-free, but they give you the best performance related information. Look for "Ration Quantity", "AQTime" and "Performance Validator" if you want a profiler of this type.
- Profilers that don't instrument the application, but just look at a running application and collect 'samples' of it. These profilers are fast (no performance loss), often easy to set up, and you can find quite some free alternatives. Look for "Very Sleepy" and "Luke Stackwalker" if you want a profiler of this type.
Although I used commercial profilers like Rational Quantity and AQTime in the past, and was very satisfied with the results, I found that the disadvantages (hard to setup, unexplainable crashes, slow performance) outweighed the advantages.
Therefore I switched to the free alternatives and I am mainly using "Very Sleepy" at this moment.