You can't use a file on the filesystem. Anyone who would be looking to fiddle/crack it will be smart enough to know how to track file access through basic, standard OSX features. So a file being added is out. Not only that but it is bad behavior to create files you don't remove when the app is uninstalled. People shouldn't have to have resources consumed after deleting your trial app.
As noted above, messing about in your bundle is a bad idea as well. This leaves you with three fundamental options.
1) Don't worry about it too much. Use a basic expiration system that uses the standard locations and methods. You can still use encryption in the data you store, but know that that too will be broken. Accept that copyright violation will occur unless your app is wholly unpopular.
2) Use a network call and do the validation on the server. This would require the app always be able to reach your service to be run. This is not a good idea in general. What if your servers are down? What if they are off-line? What if a network issue between you and them happens? All of those scenarios are going to happen. When they do you will likely lose customers unless your app requires a connection to your servers already to operate (like say Twitter or Facebook does).
3) Be a "bad citizen" by mucking around with the application bundle or leaving orphan files around. If you do this latter, at least make sure they are clearly named so that they relate to your application obviously.
Ultimately the key thing to remember is that you have no security on a user's machine. It is theirs. That means they have physical access which pretty much nullifies any attempt to prevent them from digging. You can also look at it this way: the more technically minded your market is, the less likely you are going to be smarter than all of us and your "security" will be cracked. If you are designing for a non-technical audience then you can figure that generally speaking they won't be bothering with cracking it, or looking for one.
You can spend your resources on making the app better, or giving yourself a better feeling about people not using it past the trial period. One of those can increase your sales, and one will not.
[edit]
Also I should point out that one of the most common (if not the most common) means of cracking these things is to modify the binary. So by breaking code signing via bundle mucking you would actually open yourself to that method because you would have broken one of the better protections you have. Most cracks involve binaries being modified such that the routine that does the checks always returns a successful authentication.