To answer your question in the simplest way No.
Now for the longer answer, function overhead is so small that you won't begin to notice it until you've made at least several million calls on a particular function. In most UI programs that very rarely happens having millions of calls to a method. What's inside your function is going to contribute vastly more to your performance than the overhead associated with calling the function.
To further illustrate my point. Computers operate on the nanosecond timeframe. Humans can detect about 10 milliseconds (from UI testing). 1ms = 1.0 × 10^6 ns. So if it takes 1ns to call your function you'd need to call it between 1 million to 10 million times before a human could tell the difference.
If an function's contents is expensive to call then reducing when you call it can help performance, but again that has everything to do with what's inside the function and NOT the act of calling the function.
Never try and optimize before you've measured it. I'll say it again never never try and predict what will be performant in your application. Always measure performance with a profiler. It's like trying to predict the bugs in your program before you've written it.