If you are looking at the assembly, then its fair to assume that you have a good understanding about how code gets compiled down. If you have this knowledge, then its sometimes possible to 'reverse enginer' the changes back up into the original language but its often better not to bother.
The optimisations that you make are likely to be very small in comparison to the time and effort required in first making these changes. I would suggest that you leave this kind of work to the compiler and go have a cup of tea. If the changes are significant, and the performance is critical, (as say in the embedded world) then you might want to mix the normal code with the assemblar in some fashion, however, on most computers and chips the performance is usually sufficient to avoid this headache.
If you really need more performance, then optimise the code not the assembly.