This is a good question.
Usually the best practice is 1) just write the code to do what you need it to do, 2) then deal with performance, but only if it's an issue. If the program is "fast enough" it's not an issue.
If the program is not fast enough (like it makes you wait) then try some performance tuning. Performance tuning is not like programming. In programming, you think first and then do something. In performance tuning, thinking first is a mistake, because that is guessing.
Don't guess what to fix; diagnose what the program is doing.
Everybody knows that, but mostly they do it anyway.
It is natural to say "Could be the problem is X, Y, or Z" but only the novice acts on guesses. The pro says "but I'm probably wrong".
There are different ways to diagnose performance problems.
The simplest is just to single-step through the program at the assembly-language level, and don't take any shortcuts. That way, if the program is doing unnecessary things, then you are doing the same things, and it will become painfully obvious.
Another is to get a profiling tool, and as others say, measure, measure, measure.
Personally I don't care for measuring. I think it's a fuzzy microscope for the purpose of pinpointing performance problems. I prefer this method, and this is an example of its use.
Good luck.
ADDED: I think you will find, if you go through this exercise a few times, you will learn what coding practices tend to result in performance problems, and you will instinctively avoid them. (This is subtly different from "premature optimization", which is assuming at the beginning that you must be concerned about performance. In fact, you will probably learn, if you don't already know, that premature concern about performance can well cause the very problem it seeks to avoid.)