For starters, both calls (require_once and include_once) double-check if a file has not been included before.
So the way they both achieve this is by searching the file in all available paths and by essentially checking if it hasn't been in the mix before etc..
In the background what happens is that they evaluate all the different options (e.g. multiple include_path's, etc.) and then by creating the realpath from this abreviated form they create a unique identifier. There is only one and the same path - not two.
This is already not the fasted thing on the planet and generally happens on each request with PHP. Then add another expensive operation which is the stat when it creates what I called the realpath (realpath, because it's sort of what realpath() does) to check if the file exists.
Correct me if I am wrong, but APC has optimizations especially for this case.
So anyway - now on to the difference between require_once and include_once, which is that require_once evaluates the file (for low-level parse errors, etc.) when it includes it. This is an additional check which you can get rid off if you have enough QA in place that a parse error can never sneak into an include.
It's just tricky to find otherwise. :-)
(Something to consider: You could develop with require_once and replace all calls with include_once when you deploy.)
As for an opcode cache - I'd recommend APC. It's been discussed on stackoverflow before. Personally, I am/we are using it for a while (we roughly handle 100k visitors/day with 3 frontends and 1 backend) and we are very happy. APC is also optimized for the require_once/include_once madness.
A pretty cool side-effect is that APC also allows you to store PHP variables in memory - sort of persistant, etc..
A couple additional pointers:
- A lot of people claim to speed up any application with __autoload.
- With an opcode cache, avoid conditional require_once/include_once (e.g. in loops or in control-flow).
- Some people say that /absolute/path/to/file.php in include_ or require_once is faster than relying on the include_path.
- The order of the paths in your include_path matters as well.
Hope that helps.